Journal: Cancers

Article Title: Investigating the Radiobiological Response to Peptide Receptor Radionuclide Therapy Using Patient-Derived Meningioma Spheroids

doi: 10.3390/cancers16142515

Figure Lengend Snippet: Assessment of DNA damage in meningioma spheroids after EBRT and PRRT. ( A , D ) IHC staining for SSTR2 in parental tumor tissue for a meningioma staining strongly (MO-14, ( A ) and weakly (MO-12 ( D )) SSTR2-positive. ( B , E ) Representative images of 53BP1 IF staining (red) used for the quantifications in ( C , F ). Examples are shown for EBRT (2 Gy)- and PRRT (1 MBq/mL)-treated spheroids at both day 0 and day 2 post-therapy. DAPI (blue) was used as a nuclear counterstain. ( C , F ) Quantification of the number of 53BP1 foci per nucleus after EBRT, PRRT, or PRRT co-incubated with DOTA-TATE at day 0 or day 2 post-therapy for MO-14 ( C ) and MO-12 ( F ). Black horizontal bars indicate the mean. ns = not significant; *** p ≤ 0.001; **** p ≤ 0.0001. Line graphs on the right show the mean number of 53BP1 foci for each condition. Quantification for other meningioma samples can be found in .

Article Snippet: For IF and IHC stainings, primary antibodies for SSTR2 (Abcam, Cambridge, UK; ab134152, 8.15 μg/mL), 53BP1 (Millipore, Amsterdam, The Netherlands; MAB3802, 1 μg/mL), and Ki-67 (DAKO M7240, clone MIB1, 0.48 μg/mL) were used.

Techniques: Immunohistochemistry, Staining, Incubation